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sc 516140  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology sc 516140
    Sc 516140, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sc+516140/m-IgG%CE%BA+BP-FITC/pmc13000956-109-39-40
    Average 93 stars, based on 89 article reviews
    sc 516140 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Prostaglandin F2 Alpha Triggers the Disruption of Cell Adhesion with Cytokeratin and Vimentin in Bovine Luteal Theca Cells
    Article Snippet: Samples were centrifuged at 400× g for 5 min, then samples were incubated in 1 mL 3% BSA/PBS containing β-actin mouse monoclonal IgG (1:250, sc-47778, SCBT) for 1 h at RT, after being washed twice (400× g and 5 min) using 3% BSA/PBS solution. .. The samples were incubated in 1 mL 3% BSA/PBS containing mouse-IgGκ binding protein-conjugated to fluorescein (BP-FITC) (1:250, sc-516140, SCBT) for 30 min at RT in the dark room, after being washed twice using 1% BSA/PBS solution. .. The cells were resuspended in 1 mL PBS, and analyzed via flow cytometry (FACSCalibur, BD Bioscience, San Jose, CA, USA) using argon laser tuned to 488 nm.

    Binding Assay:

    Article Title: Prostaglandin F2 Alpha Triggers the Disruption of Cell Adhesion with Cytokeratin and Vimentin in Bovine Luteal Theca Cells
    Article Snippet: Samples were centrifuged at 400× g for 5 min, then samples were incubated in 1 mL 3% BSA/PBS containing β-actin mouse monoclonal IgG (1:250, sc-47778, SCBT) for 1 h at RT, after being washed twice (400× g and 5 min) using 3% BSA/PBS solution. .. The samples were incubated in 1 mL 3% BSA/PBS containing mouse-IgGκ binding protein-conjugated to fluorescein (BP-FITC) (1:250, sc-516140, SCBT) for 30 min at RT in the dark room, after being washed twice using 1% BSA/PBS solution. .. The cells were resuspended in 1 mL PBS, and analyzed via flow cytometry (FACSCalibur, BD Bioscience, San Jose, CA, USA) using argon laser tuned to 488 nm.



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